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pGEx-4T-1-YTHDF2
(Plasmid #52301)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52301 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEx-4T-1
  • Backbone size w/o insert (bp) 4969
  • Total vector size (bp) 6538
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    For protein expression, please use BL21 and 16 C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YTHDF2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1590
  • GenBank ID
    NM_001172828
  • Entrez Gene
    YTHDF2 (a.k.a. CAHL, HGRG8, NY-REN-2)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5' GGGCTGGCAAGCCACGTTTGGTG 3'
  • 3′ sequencing primer 5' CCGGGAGCTGCATGTGTCAGAGG 3'
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEx-4T-1-YTHDF2 was a gift from Chuan He (Addgene plasmid # 52301 ; http://n2t.net/addgene:52301 ; RRID:Addgene_52301)
  • For your References section:

    N6-methyladenosine-dependent regulation of messenger RNA stability. Wang X, Lu Z, Gomez A, Hon GC, Yue Y, Han D, Fu Y, Parisien M, Dai Q, Jia G, Ren B, Pan T, He C. Nature. 2014 Jan 2;505(7481):117-20. doi: 10.1038/nature12730. Epub 2013 Nov 27. 10.1038/nature12730 PubMed 24284625