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pRB17
(Plasmid #52527)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52527 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pJet1.2
  • Backbone manufacturer
    Thermor Fisher / Fermentas
  • Backbone size w/o insert (bp) 2972
  • Total vector size (bp) 3407
  • Vector type
    Insect Expression, CRISPR ; blunt cloning kit

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Drosophila U6C promoter
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    435
  • Tag / Fusion Protein
    • T7-promoter

Cloning Information

Resource Information

Depositor Comments

Depositor states that there may are additional non-annotated NcoI sites in the backbone. Digest results may not accurately reflect full plasmid sequence provided. Depositor states that the plasmid should function normally despite this discrepancy.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRB17 was a gift from Klaus Foerstemann (Addgene plasmid # 52527 ; http://n2t.net/addgene:52527 ; RRID:Addgene_52527)
  • For your References section:

    Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells. Bottcher R, Hollmann M, Merk K, Nitschko V, Obermaier C, Philippou-Massier J, Wieland I, Gaul U, Forstemann K. Nucleic Acids Res. 2014 Apr 19. 10.1093/nar/gku289 PubMed 24748663