PurposeOpLac1 without the first 6 nucleotides of lacZ
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||52698||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert nameOpLac1-Δ6 strain
- Cloning method Unknown
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Oplac1Δ6 were erroneously synthesized missing the first 6 nucleotides of Oplac1. These deletions correspond to the first 2 N-terminal amino acid residues (Methionine and Threonine), and instead begin at the Methionine at position 3.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:OpLac1-delta-6 was a gift from Tanja Kortemme (Addgene plasmid # 52698)
For your References section:Cost-benefit tradeoffs in engineered lac operons. Eames M, Kortemme T. Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083. 10.1126/science.1219083 PubMed 22605776