PurposeKIlac with lacZ C-terminal sssA degradation tag
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Bacterial Strain||52705||Bacteria in agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Gene/Insert namedegtag strain
- Cloning method Unknown
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
To generate the degtag strain, we introduced the ssrA tag to the C-terminus of LacZ, thus targeting
the protein for degradation by the ClpXP and ClpAP proteases.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:degtag was a gift from Tanja Kortemme (Addgene plasmid # 52705)
For your References section:Cost-benefit tradeoffs in engineered lac operons. Eames M, Kortemme T. Science. 2012 May 18;336(6083):911-5. doi: 10.1126/science.1219083. 10.1126/science.1219083 PubMed 22605776