pAG25-L-IFP-F (clon 3)
PurposePlasmid template for PCR of cassettes for HR, used to introduce IFP F fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||52899||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeVector for homologous recombination
Growth in Bacteria
Copy numberHigh Copy
- Promoter TEF promoter
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer Unknown
- 3′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAG25-L-IFP-F (clon 3) was a gift from Stephen Michnick (Addgene plasmid # 52899)