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An infrared reporter to detect spatiotemporal dynamics of protein-protein interactions.
Tchekanda E, Sivanesan D, Michnick SW
Nat Methods. 2014 Jun;11(6):641-4. doi: 10.1038/nmeth.2934. Epub 2014 Apr 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
52893p413GAL1-Zipp.-L-IFP-F[2] (Yeast)Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[2] by the linker (GGGGS) X 2. Zipper-L-IFP-F[2] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.
52895p416GAL1-Zipp.-L-IFP-F[1] (Yeast)Plasmid+insert encoding GCN4-leucine zipper fused to IFP-F[1] by the linker (GGGGS) X 2. Zipper-L-IFP-F[1] (XbaI/XhoI). Zipper is (XbaI /ClaI). Plasmid confers ampicillin resistance to DH5α.
52896p416GAL1-Skn7t-L-IFP-F[1] (Yeast)Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.
52897p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.
52898p413GAL1-Ypd*1-L-IFP-F[2] (Yeast)YpdI* is fused to the IFP-F[2] by (GGGGS) X 2. Ypd1*/Skn7t: no signal, Ypd1*/Ssk1t: weak signal. Internal ClaI site in Ypd*I. Plasmid confers ampicillin resistance to DH5α.
52899pAG25-L-IFP-F[1] (clon 3)Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
52900pAG32-L-IFP-F[2] (clon 3)Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
52901pLpC-L-IFP-F[1] (mammalian)Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α.
52902pLpC-L-IFP-F[2] (mammalian)Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned betweeen the MCS. Plasmid confers ampicillin resistance to DH5α.
52903pLpC-Reg-L-IFP-F[2]+Venus (mammalian)Binary retroviral pl. (puromycin). Reg:Linker (GGGGS)X2+IFP-F[2] (PacI/EcoRI). In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
52905pLpC-Cat-L-IFP-F[1]+Plum (mammalian)Binary retroviral pl. (puromycin). Cat(PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
52906pLpC-Zipp.-L-IFP-F[2]+Venus (mammalian)Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[2]. In the second MCS, the Venus fluorescent protein is cloned in between PmeI/SalI.Confers ampicillin resistance to DH5α.
52907pLpC-Zipp.-L-IFP-F[1]+Plum (mammalian)Binary retroviral pl. (puromycin). Zipper (PacI/ClaI):Linker (GGGGS)X2+IFP-F[1]. In the second MCS, the Plum fluorescent protein is cloned in between PmeI/SalI. Confers ampicillin resistance to DH5α.

Antibodies from Article