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pZmU3-gRNA
(Plasmid #53061)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53061 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEasy-blunt
  • Backbone manufacturer
    Transgen
  • Backbone size w/o insert (bp) 3929
  • Vector type
    CRISPR ; Plant expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    zmU3 promoter - gRNA
  • Species
    maize (corn)
  • Insert Size (bp)
    869
  • Promoter maize U3

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZmU3-gRNA was a gift from Caixia Gao (Addgene plasmid # 53061 ; http://n2t.net/addgene:53061 ; RRID:Addgene_53061)
  • For your References section:

    Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System. Liang Z, Zhang K, Chen K, Gao C. J Genet Genomics. 2014 Feb 20;41(2):63-8. doi: 10.1016/j.jgg.2013.12.001. Epub 2013 Dec 14. 10.1016/j.jgg.2013.12.001 PubMed 24576457