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pAC-NEUR
(Plasmid #53271)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53271 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAC-PHYT
  • Backbone manufacturer
    Francis X. Cunningham, Jr.
  • Backbone size w/o insert (bp) 8106
  • Total vector size (bp) 11849
  • Modifications to backbone
    A 3.7 kB EcoRV-EcoRV fragment containing the phytoene desaturase gene from Rhodobacter capsulatus (crtI) was excised from plasmid GABX2 (See: Armstrong, G.A., Alberti, M., Leach, F., and Hearst, J.E. (1989). Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus. Mol. Gen. Genet. 216, 254-268.) and inserted in the Klenow-blunted SalI site of pAC-PHYT.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best neurosporene production, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    crtI
  • Alt name
    phytoene desaturase
  • Species
    Rhodobacter capsulatus
  • GenBank ID
    Z11165.1
  • Promoter endogenous promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-NEUR was a gift from Francis X Cunningham Jr (Addgene plasmid # 53271 ; http://n2t.net/addgene:53271 ; RRID:Addgene_53271)
  • For your References section:

    Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. Cunningham FX Jr, Sun Z, Chamovitz D, Hirschberg J, Gantt E. Plant Cell. 1994 Aug;6(8):1107-21. 10.1105/tpc.6.8.1107 PubMed 7919981