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pAC-BETAipi
(Plasmid #53277)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53277 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAC-BETA
  • Backbone manufacturer
    Francis X. Cunningham, Jr.
  • Backbone size w/o insert (bp) 10609
  • Total vector size (bp) 9259
  • Modifications to backbone
    A 1.6 kb SphI-DrdI fragment was deleted from pAC-BETA to give pAC-BETA9.0 (unpublished). A 2.6 kb HindIII-blunted SphI fragment, containing CrtE and idi (ORF6), a gene encoding a type 2 isopentenyl diphosphate isomerase, was excised from pAC-EHER and inserted into the HindIII-Klenow-blunted SalI sites of pAC-BETA9.0 to give pAC-BETAipi.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Top10
  • Growth instructions
    Grow liquid cultures on a platform shaker at 28 degrees Celsius for 2-3 days in darkness for best beta-carotene production, or grow on agar plates at room temperature for 3-7 days.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    idi
  • Alt name
    isopentenyl diphosphate isomerase
  • Alt name
    ORF6
  • Species
    Erwinia herbicola Eho10
  • GenBank ID
    M87280.1
  • Promoter endogenous promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-BETAipi was a gift from Francis X Cunningham Jr (Addgene plasmid # 53277 ; http://n2t.net/addgene:53277 ; RRID:Addgene_53277)
  • For your References section:

    A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant J. 2005 Feb;41(3):478-92. 10.1111/j.1365-313X.2004.02309.x PubMed 15659105