|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53368||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4361
- Total vector size (bp) 6150
Modifications to backboneAmp resistance gene and origin of replication from pBR322. MCK 1.35 promoter/enhancer can be excised with AatII or NdeI at 5', and HindIII or EcoRI at 3' end. Luciferase gene (Photinus pyralis). SV40 pA.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namemuscle creatine kinase promoter/enhancer 1.35kb
SpeciesM. musculus (mouse)
Insert Size (bp)1354
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI, AatII (not destroyed)
- 3′ cloning site HindIII, EcoRI (not destroyed)
- 5′ sequencing primer CTGTCATGCCATCCGTAAGA (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBR322-MCK1.35lux was a gift from Josephine Nalbantoglu (Addgene plasmid # 53368 ; http://n2t.net/addgene:53368 ; RRID:Addgene_53368)
For your References section:Efficient muscle-specific transgene expression after adenovirus-mediated gene transfer in mice using a 1.35 kb muscle creatine kinase promoter/enhancer. Larochelle N, Lochmuller H, Zhao J, Jani A, Hallauer P, Hastings KE, Massie B, Prescott S, Petrof BJ, Karpati G, Nalbantoglu J. Gene Ther. 1997 May;4(5):465-72. 10.1038/sj.gt.3300414 PubMed 9274724