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pSQT834
(Plasmid #53371)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 53371 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Csy4-T2A-Cas9-NLS
  • Alt name
    Csy4
  • Alt name
    Cas9
  • Species
    H. sapiens (human)
  • Promoter CAG

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSQT834 was a gift from Keith Joung (Addgene plasmid # 53371 ; http://n2t.net/addgene:53371 ; RRID:Addgene_53371)
  • For your References section:

    Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325