Purposelentiviral expression of constitutively active human Akt1 and EGFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53583||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepHR-IG (pHR’tripCMV-IRES-eGFP)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Alt namethymoma viral proto-oncogene 1
SpeciesH. sapiens (human)
Mutationconstitutively active (myristoylated version)
Entrez GeneAKT1 (a.k.a. AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
/ Fusion Proteins
- HA (C terminal on insert)
- IRES-EGFP (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
Articles Citing this Plasmid
cAkt1-HA (pcDNA3.1) provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University.
cAkt1 was fused with a Gag polypeptide and lacks the pleckstrin homology (PH) domain; the expression of a myristoylation signal enables Akt1 protein to attach to the cell membrane and become constitutively phosphorylated, thus activated (Aimee 1996).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHRIG-Akt1 was a gift from Heng Zhao (Addgene plasmid # 53583 ; http://n2t.net/addgene:53583 ; RRID:Addgene_53583)
For your References section:Akt isoforms differentially protect against stroke-induced neuronal injury by regulating mTOR activities. Xie R, Cheng M, Li M, Xiong X, Daadi M, Sapolsky RM, Zhao H. J Cereb Blood Flow Metab. 2013 Dec;33(12):1875-85. doi: 10.1038/jcbfm.2013.132. Epub 2013 Aug 14. 10.1038/jcbfm.2013.132 PubMed 23942361
Map uploaded by the depositor.