PurposeNano-lantern-based luminescent cAMP indicator
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53591||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2900
- Total vector size (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameNano-lantern-based luminescent cAMP indicator
Insert Size (bp)2100
/ Fusion Protein
- 6xHis (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BmHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Nano-lantern(cAMP-1.6)/pRSETB was a gift from Takeharu Nagai (Addgene plasmid # 53591 ; http://n2t.net/addgene:53591 ; RRID:Addgene_53591)
For your References section:Luminescent proteins for high-speed single-cell and whole-body imaging. Saito K, Chang YF, Horikawa K, Hatsugai N, Higuchi Y, Hashida M, Yoshida Y, Matsuda T, Arai Y, Nagai T. Nat Commun. 2012;3:1262. doi: 10.1038/ncomms2248. 10.1038/ncomms2248 PubMed 23232392
Map uploaded by the depositor.