Purposelentiviral expression of constitutively active PRAS40 and EGFP
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||53599||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepHR-IG (pHR’tripCMV-IRES-eGFP)
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Alt nameAKT1 substrate 1 (proline-rich)
SpeciesH. sapiens (human)
Mutationconstitutively active (myristoylated version)
Entrez GeneAKT1S1 (a.k.a. Lobe, PRAS40)
- Promoter CMV
/ Fusion Proteins
- IRES-EGFP (C terminal on insert)
- myc (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pCDH-rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
PRAS40 (pCMV5) was provided by Dr Richard Roth, Department of Chemical and Systems Biology, Stanford University.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pHRIG-PRAS40 was a gift from Heng Zhao (Addgene plasmid # 53599 ; http://n2t.net/addgene:53599 ; RRID:Addgene_53599)
For your References section:PRAS40 plays a pivotal role in protecting against stroke by linking the Akt and mTOR pathways. Xiong X, Xie R, Zhang H, Gu L, Xie W, Cheng M, Jian Z, Kovacina K, Zhao H. Neurobiol Dis. 2014 Jun;66:43-52. doi: 10.1016/j.nbd.2014.02.006. Epub 2014 Feb 27. 10.1016/j.nbd.2014.02.006 PubMed 24583056
Map uploaded by the depositor.