PurposeLocalization: Gap Junctions, Excitation: 487, Emission: 509
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54054||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameBeta-1 Connexin 32
SpeciesH. sapiens (human)
Entrez GeneGJB1 (a.k.a. CMTX, CMTX1, CX32)
- Promoter CMV
/ Fusion Protein
- mEmerald (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Human Beta-1 Connexin 32. Excitation = 487; Emission = 509. Localization data: Gap Junctions
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mEmerald-Cx32-7 was a gift from Michael Davidson (Addgene plasmid # 54054 ; http://n2t.net/addgene:54054 ; RRID:Addgene_54054)
For your References section:In vitro motility of liver connexin vesicles along microtubules utilizes kinesin motors. Fort AG, Murray JW, Dandachi N, Davidson MW, Dermietzel R, Wolkoff AW, Spray DC. J Biol Chem. 2011 Jul 1;286(26):22875-85. doi: 10.1074/jbc.M111.219709. Epub 2011 May 2. 10.1074/jbc.M111.219709 PubMed 21536677