PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||54961||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
MutationV234L in Vinculin.
Entrez GeneVCL (a.k.a. CMD1W, CMH15, HEL114, MV, MVCL)
- Promoter CMV
/ Fusion Protein
- mApple (N terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 568; Emission = 592
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mApple-Vinculin-23 was a gift from Michael Davidson (Addgene plasmid # 54961 ; http://n2t.net/addgene:54961 ; RRID:Addgene_54961)
For your References section:Vinculin-actin interaction couples actin retrograde flow to focal adhesions, but is dispensable for focal adhesion growth. Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, Waterman CM. J Cell Biol. 2013 Jul 8;202(1):163-77. doi: 10.1083/jcb.201303129. 10.1083/jcb.201303129 PubMed 23836933