PurposeStrong constitutive promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55175||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2070
- Total vector size (bp) 2227
Vector typeSynthetic Biology ; Bacillus BioBrick Box
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namelepA promoter
Insert Size (bp)157
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site PstI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
Promoter expresses highly conserved genes in bacteria and mitochondria necessary for exact protein translation.
For sequencing of insert, use the following primers:
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PlepA was a gift from Thorsten Mascher (Addgene plasmid # 55175 ; http://n2t.net/addgene:55175 ; RRID:Addgene_55175)
For your References section:The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis. Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T. J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. 10.1186/1754-1611-7-29 PubMed 24295448