The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PubMed Article
Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 55168 | pBS1C | Empty vector, integration at amyE, ampr, cmr |
| 55169 | pBS2E | Empty vector, integration at lacA, ampr, mlsr |
| 55170 | pBS4S | Empty vector, integration at thrC, ampr, specr |
| 55171 | pBS1ClacZ | lacZ-reporter vector, integration at amyE, ampr, cmr |
| 55172 | pBS3Clux | lux-reporter vector, integration at sacA, ampr, cmr |
| 55173 | Pveg | Very strong constitutive promoter |
| 55174 | PliaG | Constitutive promoter |
| 55175 | PlepA | Strong constitutive promoter |
| 55176 | J23101 | Very weak constitutive promoter |
| 55177 | PliaI | Bacitracin-inducible promoter |
| 55178 | PxylA | Xylose-inducible promoter |
| 55179 | His10-tag | 10xHis-tag |
| 55180 | FLAG-tag | FLAG-tag |
| 55181 | StrepII-tag | Streptactin-tag |
| 55182 | HA-tag | HA-tag |
| 55183 | cMyc-tag | cMyc tag |