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The Bacillus BioBrick Box: generation and evaluation of essential genetic building blocks for standardized work with Bacillus subtilis.
Radeck J, Kraft K, Bartels J, Cikovic T, Durr F, Emenegger J, Kelterborn S, Sauer C, Fritz G, Gebhard S, Mascher T
J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.1186/1754-1611-7-29.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
55168pBS1CEmpty vector, integration at amyE, ampr, cmr
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55169pBS2EEmpty vector, integration at lacA, ampr, mlsr
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55170pBS4SEmpty vector, integration at thrC, ampr, specr
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55171pBS1ClacZlacZ-reporter vector, integration at amyE, ampr, cmr
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55172pBS3Cluxlux-reporter vector, integration at sacA, ampr, cmr
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55173PvegVery strong constitutive promoter
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55174PliaGConstitutive promoter
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55175PlepAStrong constitutive promoter
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55176J23101Very weak constitutive promoter
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55177PliaIBacitracin-inducible promoter
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55178PxylAXylose-inducible promoter
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55179His10-tag10xHis-tag
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55180FLAG-tagFLAG-tag
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55181StrepII-tagStreptactin-tag
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55182HA-tagHA-tag
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55183cMyc-tagcMyc tag
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