Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pGreen_dualluc_3'UTR_sensor
(Plasmid #55206)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55206 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGreen II
  • Backbone manufacturer
    pGreen II
  • Backbone size w/o insert (bp) 2500
  • Total vector size (bp) 7070
  • Vector type
    Luciferase ; Plant transient expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Renilla luciferase
  • Alt name
    R-Luc
  • Species
    Renilla reniformis
  • Insert Size (bp)
    936

Gene/Insert 2

  • Gene/Insert name
    Firefly luciferase
  • Alt name
    F-Luc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1653

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGreen_dualluc_3'UTR_sensor was a gift from Michael Axtell (Addgene plasmid # 55206 ; http://n2t.net/addgene:55206 ; RRID:Addgene_55206)
  • For your References section:

    Analysis of complementarity requirements for plant MicroRNA targeting using a Nicotiana benthamiana quantitative transient assay. Liu Q, Wang F, Axtell MJ. Plant Cell. 2014 Feb;26(2):741-53. doi: 10.1105/tpc.113.120972. Epub 2014 Feb 7. 10.1105/tpc.113.120972 PubMed 24510721