pGreen_dualluc_ORF_sensor
(Plasmid #55207)

• Purpose: Dual-luciferase reporter for microRNA targeting in plants
• Depositing Lab: Michael Axtell
• Publication: Liu et al Plant Cell. 2014 Feb;26(2):741-53. doi: 10.1105/tpc.113.120972. Epub 2014 Feb 7.

Backbone

• Vector backbone: pGreen II
• Backbone manufacturer: pGreen II
• Backbone size w/o insert (bp): 2500
• Total vector size (bp): 7067
• Vector type: Luciferase ; Plant transient expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Renilla luciferase
• Alt name: R-Luc
• Species: Renilla reniformis
• Insert Size (bp): 936

Gene/Insert 2

• Gene/Insert name: Firefly luciferase
• Alt name: F-Luc
• Species: Photinus pyralis
• Insert Size (bp): 1653

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Modified from on pGreen II 0229 and pGreen II 0000
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry