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(Plasmid #55594)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 55594 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pBluescript II SK+
  • Backbone size (bp) 2900
  • Vector type
    Mammalian Expression
  • Promoter alpha MHC

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJG/ALPHA MHC was a gift from Jeffrey Robbins (Addgene plasmid # 55594 ; ; RRID:Addgene_55594)
  • For your References section:

    Isolation and characterization of the mouse cardiac myosin heavy chain genes. Gulick J, Subramaniam A, Neumann J, Robbins J. J Biol Chem. 1991 May 15;266(14):9180-5. PubMed 2026617