pJG/ Inducible ALPHA MHC
Purpose(Empty Backbone) transgene expression driven by inducible ALPHA MHC promoter
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||55792||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript II SK+
Vector typeMammalian Expression
- Promoter Inducible ALPHA MHC
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
The distance between the two Not1 sites is ~6kb
Clone into the Sal1 site (or Sal/Hind3 site).
Release the pBluescript backbone with Not1 for transgenics.
This construct consists of a full-length α-MHC promoter in which 3 GATA sites and 2 TREs were ablated. Other cis-acting regions important for cardiac-specific expression were left intact. To make the attenuated promoter Tet responsive, 7 repeats of the TetO sequences were inserted adjacent to the TATA box. The human growth hormone polyadenylation signal was placed downstream from the unique cloning site. Any gene of interest inserted into this Tet-responsive promoter construct will be inducible.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJG/ Inducible ALPHA MHC was a gift from Jeffrey Robbins (Addgene plasmid # 55792 ; http://n2t.net/addgene:55792 ; RRID:Addgene_55792)
For your References section:Reengineering inducible cardiac-specific transgenesis with an attenuated myosin heavy chain promoter. Sanbe A, Gulick J, Hanks MC, Liang Q, Osinska H, Robbins J. Circ Res. 2003 Apr 4;92(6):609-16. Epub 2003 Mar 6. 10.1161/01.RES.0000065442.64694.9F PubMed 12623879
Map uploaded by the depositor.