Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

G protein alpha-q-YFP
(Plasmid #55782)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 55782 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA1/amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7120
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-q EE YFP
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2319
  • Mutation
    BamHI and SacI sites in alpha-q were removed with silent mutations. EYFP contains substitution of Met for Gln69.
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk, Dsk1, Dsk10, Gal, Galphaq, Gq, GqI)
  • Promoter CMV
  • Tags / Fusion Proteins
    • YFP was inserted internally into the alpha-q sequence between residues 124 and 125 with a SGGGGS linker on each end of the YFP.
    • The EE epitope was introduced into the alpha-q sequence by mutating residues 171-176 (SYLPTQ) to EYMPTE.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-q-YFP was a gift from Catherine Berlot (Addgene plasmid # 55782 ; http://n2t.net/addgene:55782 ; RRID:Addgene_55782)
  • For your References section:

    Live cell analysis of G protein beta5 complex formation, function, and targeting. Yost EA, Mervine SM, Sabo JL, Hynes TR, Berlot CH. Mol Pharmacol. 2007 Oct;72(4):812-25. Epub 2007 Jun 27. 10.1124/mol.107.038075 PubMed 17596375