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G protein alpha-q-CFP
(Plasmid #66081)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 66081 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNAI/Amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 7119
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein alpha-q-EE-CFP
  • Alt name
    Gnaq
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2319
  • Mutation
    BamHI and SacI sites in alpha-q were removed with silent mutations. EYFP contains substitution of His for Asn164.
  • Entrez Gene
    Gnaq (a.k.a. 1110005L02Rik, 6230401I02Rik, AA408290, AW060788, Dsk1, Dsk10, Galphaq, Gq, GqI)
  • Promoter CMV
  • Tags / Fusion Proteins
    • CFP was inserted internally into the alpha-q sequence between residues 124 and 125 with a SGGGGS linker on each end of the YFP.
    • The EE epitope was introduced into the alpha-q sequence by mutating residues 171-176 (SYLPTQ) to EYMPTE.

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G protein alpha-q-CFP was a gift from Catherine Berlot (Addgene plasmid # 66081 ; http://n2t.net/addgene:66081 ; RRID:Addgene_66081)
  • For your References section:

    Visualization of a functional Galpha q-green fluorescent protein fusion in living cells. Association with the plasma membrane is disrupted by mutational activation and by elimination of palmitoylation sites, but not be activation mediated by receptors or AlF4-. Hughes TE, Zhang H, Logothetis DE, Berlot CH. J Biol Chem. 2001 Feb 9;276(6):4227-35. Epub 2000 Nov 13. 10.1074/jbc.M007608200 PubMed 11076942