PurposeLocalization: Microtubules, Excitation: 549, Emission: 565
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||57974||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4750
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneTUBA1B (a.k.a. K-ALPHA-1)
- Promoter CMV
/ Fusion Protein
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
. Excitation = 549; Emission = 565
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mOrange2-Tubulin-6 was a gift from Michael Davidson (Addgene plasmid # 57974 ; http://n2t.net/addgene:57974 ; RRID:Addgene_57974)
For your References section:Improving the photostability of bright monomeric orange and red fluorescent proteins. Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson MW, Tsien RY. Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 2008 May 4. 10.1038/nmeth.1209 PubMed 18454154