|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58303||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Growth Strain(s)E. coli MC1061 DIAL EI ΔdapA-Kis
Growth instructionsFor plasmid propagation, a host with an integrated GeneGuard genomic cassette is required.
A portion of this plasmid was derived from a plasmid made byKid/Kis. Toxin-Antitoxin parts. Part source: Prof. Ramoń Diáz-Orejas (Dept of Molecular Microbiology and Infection Biology, Centro de Investigaciones Bioloǵicas, Madrid);DIAL strains. Part source: Prof J. Christopher Anderson (Dept of Bioengineering, University of California, Berkeley);COR parts. Part source: Prof Vićtor de Lorenzo (Systems Biology Program, Centro Nacional de Biotecnologiá, Madrid)
Terms and Licenses
- Not Available to Industry
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSEVAD7K1A was a gift from Tom Ellis (Addgene plasmid # 58303 ; http://n2t.net/addgene:58303 ; RRID:Addgene_58303)
For your References section:GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673