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GeneGuard: a Modular Plasmid System Designed for Biosafety.
Wright O, Delmans M, Stan GB, Ellis T
ACS Synth Biol. 2014 May 13.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
58296pSEVAT1Z1AGeneGuard vector cassette: thyA, R6K COR, Zeta toxin; with ampR as cargo.
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58297pSEVAD1Z1AGeneGuard vector cassette: dapA, R6K COR, Zeta toxin; with ampR as cargo.
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58298pSEVAT1K1AGeneGuard vector cassette: thyA, R6K COR, Kid toxin; with ampR as cargo.
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58299pSEVAD1K1AGeneGuard vector cassette: dapA, R6K COR, Kid toxin; with ampR as cargo.
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58300pSEVAT7Z1AGeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.
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58301pSEVAD7Z1AGeneGuard vector cassette: dapA, ColE2 COR, Zeta toxin; with ampR as cargo.
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58302pSEVAT7K1AGeneGuard vector cassette: thyA, ColE2 COR, Kid toxin; with ampR as cargo.
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58303pSEVAD7K1AGeneGuard vector cassette: dapA, ColE2 COR, Kid toxin; with ampR as cargo.
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58304pSEVA12DKR-AGeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.
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58305pSEVA12DKR-EGeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.
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58306pSEVA12DKR-JGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.
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58307pSEVA12TEC-BGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.
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58308pSEVA12TEC-IGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.
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58310pSEVA3b61Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
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58311pSEVA3b67RbShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
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