pDDGFP2_leu2d_NRT1.1
(Plasmid #58332)

• Purpose: Expresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged protein
• Depositing Lab: Simon Newstead
• Publication: Parker et al Nature. 2014 Mar 6;507(7490):68-72. doi: 10.1038/nature13116. Epub 2014 Feb 26.

Backbone

• Vector backbone: pDDGFP2_LEU2D
• Backbone size w/o insert (bp): 8700
• Total vector size (bp): 10500
• Vector type: Yeast Expression
• Selectable markers: LEU2, URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NRT1.1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1800
• Entrez Gene: NRT1.1 (a.k.a. AT1G12110, ARABIDOPSIS THALIANA NITRATE TRANSPORTER 1, ATNRT1, ATNRT1.1, AtNPF6.3, B-1, CHL1, CHL1-1, CHLORATE/NITRATE TRANSPORTER, CHLORINA 1, F12F1.1, F12F1_1, NITRATE TRANSPORTER 1, NITRATE/CHLORATE TRANSPORTER, NPF6.3, NRT1, NRT1/ PTR family 6.3, nitrate transporter 1.1)
• Promoter: GAL1
• Tag / Fusion Protein:
  • GFP-His (C terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CTATACTTTAACGTCAAGG
• 3′ sequencing primer: CAGTGAATAATTCTTCACC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry