PurposeAAV-mediated expression of mCherry under the GFAP promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58909||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
|AAV5||58909-AAV5||Virus (100 µL at titer ≥ 1×10¹³ vg/mL)|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4895
- Total vector size (bp) 5606
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsStbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
Copy numberLow Copy
Insert Size (bp)711
- Promoter GFAP
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ggagagctctccccatag
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
The depositing lab has sequenced the 5' ITR, promoter, and fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.
Information for AAV5 (Catalog # 58909-AAV5) ( Back to top )
Ready-to-use AAV5 particles produced from pAAV-GFAP104-mCherry (#58909). In addition to the viral particles, you will also receive purified pAAV-GFAP104-mCherry plasmid DNA.GFAP104-driven mCherry expression control. These AAV preparations are suitable purity for injection into animals.
- Volume 100 µL
- Titer ≥ 1×10¹³ vg/mL
- Pricing $350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- Storage Store at -80℃. Thaw just before use and keep on ice.
- Shipment Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmids encode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- Buffer PBS + 0.001% Pluronic F-68 + 200 mM NaCl
- Serotype AAV5
- Purification Iodixanol gradient ultracentrifugation
- Reporter Gene mCherry
Requestor is responsible for compliance with their institution's biosafety regulations. Lentivirus is generally considered BSL-2. AAV is generally considered BSL-1, but may require BSL-2 handling depending on the insert. Biosafety Guide
Terms and Licenses
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). The specific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-GFAP104-mCherry was a gift from Edward Boyden (Addgene plasmid # 58909 ; http://n2t.net/addgene:58909 ; RRID:Addgene_58909)
For viral preps, please replace (Addgene plasmid # 58909) in the above sentence with: (Addgene viral prep # 58909-AAV5)
For your References section:Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo. Perea G, Yang A, Boyden ES, Sur M. Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262. 10.1038/ncomms4262 PubMed 24500276