PurposeAAV-mediated expression of ChR2-mCherry under the GFAP promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||58892||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4895
- Total vector size (bp) 6545
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsStbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
Copy numberLow Copy
Insert Size (bp)1650
- Promoter GFAP104
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer ggagagctctccccatag
- 3′ sequencing primer cagcgtatccacatagcg (Common Sequencing Primers)
The depositing lab has sequenced the 5' ITR, promoter, and gene-fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-GFAP104-ChR2-mCherry was a gift from Edward Boyden (Addgene plasmid # 58892)
For your References section:Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo. Perea G, Yang A, Boyden ES, Sur M. Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262. 10.1038/ncomms4262 PubMed 24500276