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pAAV-GFAP104-ChR2-mCherry
(Plasmid #58892)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58892 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    AAV
  • Backbone size w/o insert (bp) 4895
  • Total vector size (bp) 6545
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Stbl3 at 30C (use carbenicillin if using Stbl3) OR DH5a at 37C
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ChR2-mCherry
  • Alt name
    channelrhodopsin-2-mCherry
  • Species
    Chlamydomonas reinhardtii
  • Insert Size (bp)
    1650
  • GenBank ID
    EU714030.1
  • Promoter GFAP104
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer ggagagctctccccatag
  • 3′ sequencing primer cagcgtatccacatagcg
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The depositing lab has sequenced the 5' ITR, promoter, and gene-fluorophore. Multiple digestions were done to verify the vector structure. The construct was tested in vitro and the virus was tested both in vitro and in vivo.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-GFAP104-ChR2-mCherry was a gift from Edward Boyden (Addgene plasmid # 58892)
  • For your References section:

    Optogenetic astrocyte activation modulates response selectivity of visual cortex neurons in vivo. Perea G, Yang A, Boyden ES, Sur M. Nat Commun. 2014;5:3262. doi: 10.1038/ncomms4262. 10.1038/ncomms4262 PubMed 24500276