|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27055||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6290
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth instructionsPlease use Rec A- competent cells such as Stbl3 cells from Invitrogen for transformation.
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1647
/ Fusion Protein
- mCherry (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI, SalI (not destroyed)
- 3′ cloning site EcoRI, HinDIII (not destroyed)
- 5′ sequencing primer ccaaccccaccccctcaggctatg
- 3′ sequencing primer GCAATAGCATGATACAAAGG (Common Sequencing Primers)
This plasmid contains the human GFAP promoter.
For additional information please visit - http://www.optogenetics.org
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-GFAP-hChR2(H134R)-mCherry was a gift from Karl Deisseroth (Addgene plasmid # 27055 ; http://n2t.net/addgene:27055 ; RRID:Addgene_27055)