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T7-VEE-OKS-iG
(Plasmid #58974)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58974 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    T7-VEE-IRES-puro (Addgene plasmid 58970)
  • Backbone manufacturer
    Dowdy lab
  • Vector type
    Mammalian Expression ; Venezuelan equine encephalitis (VEE) virus RNA replicon
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Oct4
  • Species
    H. sapiens (human)
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4)

Gene/Insert 2

  • Gene/Insert name
    Klf4
  • Alt name
    Kruppel-like factor 4
  • Species
    H. sapiens (human)
  • Entrez Gene
    KLF4 (a.k.a. EZF, GKLF)

Gene/Insert 3

  • Gene/Insert name
    Sox2
  • Alt name
    SRY (sex determining region Y)-box 2
  • Species
    H. sapiens (human)
  • Entrez Gene
    SOX2 (a.k.a. ANOP3, MCOPS3)

Gene/Insert 4

  • Gene/Insert name
    GLIS
  • Alt name
    GLIS family zinc finger 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    GLIS1

Resource Information

Depositor Comments

Summary of features:
1-7561: VEE RNA replicases (nsP1, nsP2, nsP3, nsP4 and 26S promoter)
7592-8671: human Oct4
8678-8731: T2A peptide sequence
8738-10147: human Klf4
10154-10213: E2A peptide sequence
10223-11176: human Sox2
11195-11805: IRES sequence
11818-13680: human Glis1
13689-14300: IRES sequence
14301-14900: Puromycin resistance gene
14907-15034: VEE 3’UTR and poly A tail
15203-16063: Ampicillin resistance gene
16843-16860: T7 promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    T7-VEE-OKS-iG was a gift from Steven Dowdy (Addgene plasmid # 58974 ; http://n2t.net/addgene:58974 ; RRID:Addgene_58974)
  • For your References section:

    Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086