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Purposeexpresses B18R protein from Western vaccinia virus that binds to and neutralizes type I IFNs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58978 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTNT
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2871
- Total vector size (bp) 3907
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameB18R
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Alt nameankyrin repeat protein
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SpeciesVaccinia Virus
- Promoter T7/Sp6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MfeI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
B18R from Addgene plasmid #12794
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTNT-B18R was a gift from Steven Dowdy (Addgene plasmid # 58978 ; http://n2t.net/addgene:58978 ; RRID:Addgene_58978) -
For your References section:
Efficient generation of human iPSCs by a synthetic self-replicative RNA. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 10.1016/j.stem.2013.06.001. 10.1016/j.stem.2013.06.001 PubMed 23910086