Human SOX17 sgRNA
(Plasmid #59725)

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Purpose

Plasmid encoding sgRNA to generate SOX17 knock-out mutant human cells
Depositing Lab

Jacob Hanna
Publication

Naoko et al Cell 160, 1–16, January 15, 2015 ( How to cite )
Sequence Information
- Depositor Sequences: Partial (1)
- Addgene Sequences: Partial (1)

Full plasmid sequence is not available for this item.

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	59725	Plasmid sent as bacteria in agar stab	1	$65	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
px330
(Search Vector Database)
Backbone manufacturer
Feng Zhang
Vector type
Mammalian Expression, CRISPR

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
hSox17 sgRNA
Species
H. sapiens (human)
Insert Size (bp)
20

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site BbsI (destroyed during cloning)
3′ cloning site BbsI (destroyed during cloning)
5′ sequencing primer TGGCCTTTTGCTCACATGTG

(Common Sequencing Primers)

Resource Information

Terms and Licenses
- UBMTA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

Human SOX17 sgRNA was a gift from Jacob Hanna (Addgene plasmid # 59725)
For your References section:

SOX17 Is a Critical Specifier of Human Primordial Germ Cell Fate. Naoko Irie, Leehee Weinberger, Walfred W.C. Tang, Toshihiro Kobayashi, Sergey Viukov, Yair S. Manor, Sabine Dietmann, Jacob H. Hanna, M. Azim Surani. Cell 160, 1–16, January 15, 2015 10.1016/j.cell.2014.12.013

Human SOX17 sgRNA (Plasmid #59725)