PurposeU6 promoter driving Ebf.774 (F+E) sgRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||59990||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Modifications to backboneMutated BsaI sites in backbone
Growth in Bacteria
Gene/Insert nameEbf.774 (F+E) sgRNA
- Promoter Ciinte.U6
- Cloning method Restriction Enzyme
- 5′ cloning site BsaI (destroyed during cloning)
- 3′ cloning site BsaI (destroyed during cloning)
- 5′ sequencing primer cccaagcgagtgtttgttac
- 3′ sequencing primer GGATTTCCTTACGCGAAATACG (Common Sequencing Primers)
U6 promoter based on Nishiyama, A. and Fujiwara, S. (2008). RNA interference by expressing short hairpin RNA in the Ciona intestinalis embryo. Dev. growth … 521–529.
sgRNA F+E scaffold based on Chen, B., Gilbert, L. a, Cimini, B. a, Schnitzbauer, J., Zhang, W., Li, G.-W., Park, J., Blackburn, E. H., Weissman, J. S., Qi, L. S., et al. (2013). Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system. Cell 155, 1479–91.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:U6>Ebf.774(F+E) was a gift from Lionel Christiaen (Addgene plasmid # 59990 ; http://n2t.net/addgene:59990 ; RRID:Addgene_59990)
For your References section:Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9. Stolfi A, Gandhi S, Salek F, Christiaen L. Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488. 10.1242/dev.114488 PubMed 25336740