PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60075||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerJoung lab (Addgene plasmid # 53370)
Vector typeMammalian Expression, CRISPR
Growth in Bacteria
Growth Strain(s)XL1 Blue
Copy numberHigh Copy
Gene/Insert namepair of multiplex gRNAs targeting EGFP
Alt nameEGFP gRNA pair #83
- Promoter U6
- Cloning method Unknown
- 5′ sequencing primer OS280 (5’-CAGGGTTATTGTCTCATGAGCGG-3’) (Common Sequencing Primers)
Terms and Licenses
Left gRNA target site: AGGAGGACGGCAACATCCTG
Right gRNA target site: CAGCTCGATGCGGTTCACCA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:RFN_EGFP_83 was a gift from Keith Joung (Addgene plasmid # 60075 ; http://n2t.net/addgene:60075 ; RRID:Addgene_60075)
For your References section:Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing. Tsai SQ, Wyvekens N, Khayter C, Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Apr 25. doi: 10.1038/nbt.2908. 10.1038/nbt.2908 PubMed 24770325