PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60567||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 4338
Vector typeBacterial Expression
Growth in Bacteria
- Promoter BBa_J23119
- Cloning method Unknown
- 5′ sequencing primer GFP-F
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Terms and Licenses
This reporter plasmid contains the attB and attP sites flanking a green fluorescent protein reporter gene (gfp). A strong constitutive promoter (BBa_J23119) is placed upstream of the attB site, which transcribes in the opposite orientation as gfp. After the integrase is expressed, the orientation of gfp is inverted and it is transcribed. After inversion, recombined attB and attP sites result in the formation of attL and attR. The attL site is located on the 5'UTR and could impact gfp expression. To insulate against this effect, we included a spacer and the ribozyme RiboJ.
Also contains mRFP in order to aid the gating of cells.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pReporter_7 was a gift from Christopher Voigt (Addgene plasmid # 60567 ; http://n2t.net/addgene:60567 ; RRID:Addgene_60567)
For your References section:Permanent genetic memory with >1-byte capacity. Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu TK, Voigt CA. Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 2014 Oct 26. 10.1038/nmeth.3147 PubMed 25344638