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pHL-H1-ccdB-mEF1a-RiH
(Plasmid #60601)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60601 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHL-MCS1
  • Backbone manufacturer
    Hotta Lab, CiRA
  • Backbone size w/o insert (bp) 2043
  • Total vector size (bp) 6946
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Growth instructions
    ccdB resistant strain of E.coli is required.
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Red Fluorescent Protein
  • Alt name
    mRFP1
  • Species
    Discosoma sp
  • Insert Size (bp)
    678

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site BlnI (not destroyed)
  • 5′ sequencing primer N.A.
  • 3′ sequencing primer N.A.
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Hygromycin resistance gene
  • Alt name
    HygroR
  • Species
    E.coli
  • Insert Size (bp)
    1026

Cloning Information for Gene/Insert 2

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHL-H1-ccdB-mEF1a-RiH was a gift from Akitsu Hotta (Addgene plasmid # 60601 ; http://n2t.net/addgene:60601 ; RRID:Addgene_60601)
  • For your References section:

    Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9. Li HL, Fujimoto N, Sasakawa N, Shirai S, Ohkame T, Sakuma T, Tanaka M, Amano N, Watanabe A, Sakurai H, Yamamoto T, Yamanaka S, Hotta A. Stem Cell Reports. 2014 Nov 25. pii: S2213-6711(14)00335-X. doi: 10.1016/j.stemcr.2014.10.013. 10.1016/j.stemcr.2014.10.013 PubMed 25434822