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pCMV5-25BoxB
(Plasmid #60817)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60817 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    Dr. David Russell, UTSW
  • Backbone size w/o insert (bp) 4657
  • Total vector size (bp) 5639
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    25x BoxB
  • Alt name
    binding site of bacteriophage λ antitermination protein N
  • Alt name
    boxb
  • Species
    Synthetic; bacteriophage λ
  • Insert Size (bp)
    1042
  • Mutation
    25 repeats of BoxB sequence
  • GenBank ID
    NC_001416.1 1QFQ_A
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    A construct with 5x boxb repeats for tethering of RNA was described in Gehring et al. Mol Cell. 2003 Apr;11(4):939-49. The authors provided us with the original published reporter gene containing 5x boxb. From this precursor the 5x boxb sequence was amplified by PCR, cloned into a new vector backbone pCMV5 and multiplied to finally contain 25x boxb sequence repeats by us.
  • Terms and Licenses
  • Article Citing this Plasmid

Depositor Comments

the authors of Gehring et al. Mol Cell. 2003 Apr;11(4):939-49 were contacted by us and granted their permission to distribute the 25xboxb construct generated in our lab from their precursor for non-profit purpose via Addgene

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-25BoxB was a gift from Maria Carmo-Fonseca (Addgene plasmid # 60817 ; http://n2t.net/addgene:60817 ; RRID:Addgene_60817)
  • For your References section:

    Live-cell visualization of pre-mRNA splicing with single-molecule sensitivity. Martin RM, Rino J, Carvalho C, Kirchhausen T, Carmo-Fonseca M. Cell Rep. 2013 Sep 26;4(6):1144-55. doi: 10.1016/j.celrep.2013.08.013. Epub 2013 Sep 12. 10.1016/j.celrep.2013.08.013 PubMed 24035393