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YFP(1-158) in pcDNAI/Amp
(Plasmid #60888)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60888 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA1/amp
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Total vector size (bp) 5245
  • Modifications to backbone
    BglII site introduced into the polylinker 3' to the BamHI site
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    YFP(1-158)
  • Species
    Aequorea victoria
  • Insert Size (bp)
    445
  • Mutation
    Met was substituted for Gln-69.
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    YFP(1-158) was amplified by PCR from EYFP (Clontech)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YFP(1-158) in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 60888 ; http://n2t.net/addgene:60888 ; RRID:Addgene_60888)
  • For your References section:

    Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH. J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. 10.1074/jbc.M401432200 PubMed 15136579