YFP(1-158) in pcDNAI/Amp
PurposeThis vector attaches YFP(1-158) to the N-terminus of a protein.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60888||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4800
- Total vector size (bp) 5245
Modifications to backboneBglII site introduced into the polylinker 3' to the BamHI site
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Insert Size (bp)445
MutationMet was substituted for Gln-69.
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site BglII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YFP(1-158) in pcDNAI/Amp was a gift from Catherine Berlot (Addgene plasmid # 60888 ; http://n2t.net/addgene:60888 ; RRID:Addgene_60888)
For your References section:Visualization of G protein betagamma dimers using bimolecular fluorescence complementation demonstrates roles for both beta and gamma in subcellular targeting. Hynes TR, Tang L, Mervine SM, Sabo JL, Yost EA, Devreotes PN, Berlot CH. J Biol Chem. 2004 Jul 16;279(29):30279-86. Epub 2004 May 10. 10.1074/jbc.M401432200 PubMed 15136579