Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

(Plasmid #60900)


Item Catalog # Description Quantity Price (USD)
Plasmid 60900 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pUC ori vector
  • Backbone size (bp) 8506
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter CBh and U6
  • Tag / Fusion Protein
    • 3XFLAG (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid is generated by introduction of the H840A mutation into pX330 from the Zhang laboratory.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid

Depositor Comments

The gRNA cloning strategy is exactly the same as for pX330/pX335.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXCas9H840A was a gift from Bruce Conklin (Addgene plasmid # 60900 ; ; RRID:Addgene_60900)
  • For your References section:

    Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. Miyaoka et al. Scientific Reports 6, Article number: 23549 (2016) 10.1038/srep23549