Purpose(Empty Backbone) Dual Expression Vector for Cas9 H840A nickase and gRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60900||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC ori vector
- Backbone size (bp) 8506
Vector typeMammalian Expression, CRISPR
- Promoter CBh and U6
/ Fusion Protein
- 3XFLAG (N terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer ACTATCATATGCTTACCGTAAC (Common Sequencing Primers)
The gRNA cloning strategy is exactly the same as for pX330/pX335.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXCas9H840A was a gift from Bruce Conklin (Addgene plasmid # 60900 ; http://n2t.net/addgene:60900 ; RRID:Addgene_60900)
For your References section:Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. Miyaoka et al. Scientific Reports 6, Article number: 23549 (2016) 10.1038/srep23549