Purpose(Empty Backbone) Dual Expression Vector for FokI-dCas9 and gRNA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60901||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepUC ori vector
- Backbone size (bp) 9070
Vector typeMammalian Expression, CRISPR
- Promoter CBh and U6
/ Fusion Protein
- 3XFLAG (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer ACTATCATATGCTTACCGTAAC (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe plasmid is generated by replacing Cas9 D10A nickase in pX335 from the Zhang laboratory with FokI-dCas9 from pSQT1601 from the Joung laboratory.
Terms and Licenses
- Not Available to Industry
Articles Citing this Plasmid
The gRNA cloning strategy is exactly the same as for pX330/pX335.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pXFokI-dCas9 was a gift from Bruce Conklin (Addgene plasmid # 60901 ; http://n2t.net/addgene:60901 ; RRID:Addgene_60901)
For your References section:Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing. Miyaoka et al. Scientific Reports 6, Article number: 23549 (2016) 10.1038/srep23549