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pPL5071_TEF1*-Cre_URA3
(Plasmid #60930)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60930 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cre
  • Alt name
    Cre-recombinase
  • Species
    E. coli
  • Insert Size (bp)
    1032
  • Promoter mutant TEF1
  • Tag / Fusion Protein
    • Driven from a mutant TEF1 promoter with 0.16x activity

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer cgataccgtcgaggggcagagcc
  • 3′ sequencing primer catattttccgtcgatgttgaaatcc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    They were gene synthesized using a commercial service, which made them according to our design.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPL5071_TEF1*-Cre_URA3 was a gift from Robert Piper (Addgene plasmid # 60930 ; http://n2t.net/addgene:60930 ; RRID:Addgene_60930)
  • For your References section:

    Puromycin and Methotrexate Resistance Cassettes and Optimized cre-recombinase Expression Plasmids for use in Yeast. MacDonald C, Piper RC. Yeast. 2015 Feb 16. doi: 10.1002/yea.3069. 10.1002/yea.3069 PubMed 25688547