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p3XFlag-CAPN1
(Plasmid #60941)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 60941 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p3xFlag-CMV-14
  • Backbone manufacturer
    sigma
  • Backbone size w/o insert (bp) 6310
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Calpain1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2145
  • Entrez Gene
    CAPN1 (a.k.a. CANP, CANP1, CANPL1, SPG76, muCANP, muCL)
  • Promoter CMV
  • Tag / Fusion Protein
    • flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer CMV pro
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFlag-CAPN1 was a gift from Yi Zhang (Addgene plasmid # 60941 ; http://n2t.net/addgene:60941 ; RRID:Addgene_60941)
  • For your References section:

    Regulation of TET protein stability by calpains. Wang Y, Zhang Y. Cell Rep. 2014 Jan 30;6(2):278-84. doi: 10.1016/j.celrep.2013.12.031. Epub 2014 Jan 9. 10.1016/j.celrep.2013.12.031 PubMed 24412366