PurposeDrosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||60944||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerThermo Scientific
- Backbone size w/o insert (bp) 2974
- Total vector size (bp) 4785
Growth in Bacteria
Copy numberHigh Copy
- Promoter 3XP3
- Cloning method Unknown
- 5′ sequencing primer cgactcactatagggagagcggc
- 3′ sequencing primer aagaacatcgattttccatggcag (Common Sequencing Primers)
The plasmid contains an IS4-like element that does not interfere with function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pJET1.2-STOP-dsRed was a gift from Frank Schnorrer (Addgene plasmid # 60944 ; http://n2t.net/addgene:60944 ; RRID:Addgene_60944)
For your References section:A Versatile Two-Step CRISPR- and RMCE-Based Strategy for Efficient Genome Engineering in Drosophila. Zhang X, Koolhaas WH, Schnorrer F. G3 (Bethesda). 2014 Oct 15. pii: g3.114.013979. doi: 10.1534/g3.114.013979. 10.1534/g3.114.013979 PubMed 25324299