Purpose(Empty Backbone) Small IncP-based broad host range replicating vector.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61263||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size (bp) 4979
Modifications to backboneMinimized vector size by removing fragments of tet and bla antibiotic resistance genes left over from restriction based cloning.
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Gibson Cloning
In order to use plasmid pAWP78, inserts should be added by Gibson Cloning. Primers to amplify the backbone have been annotated on the depositor's plasmid map. The sequences are (5' to 3'):
AP259_pAWP78_fwd1 - TTGTCGGGAAGATGCGTGAT
AP254_pAWP78_rev1 - CAGCTCACTCAAAGGCGGTA
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAWP78 was a gift from Mary Lidstrom (Addgene plasmid # 61263 ; http://n2t.net/addgene:61263 ; RRID:Addgene_61263)
For your References section:Genetic tools for the industrially promising methanotroph Methylomicrobium buryatense. Puri AW, Owen S, Chu F, Chavkin T, Beck DA, Kalyuzhnaya MG, Lidstrom ME. Appl Environ Microbiol. 2014 Dec 29. pii: AEM.03795-14. 10.1128/AEM.03795-14 PubMed 25548049