Purposedrives luciferase via mouse IL-6 promoter, truncated (-231)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||61287||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepGL2 basic
- Total vector size (bp) 6877
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Gene/Insert nameTruncated IL-6 promoter
SpeciesM. musculus (mouse)
MutationDeletion of 1046 nucleotides from the 5' end of the IL-6 promoter (-231 from start site)
- Promoter IL-6 promoter (truncated)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer EBV-rev
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Full sequence and map is available for wild-type vector pmIL-6 FL (Plasmid #61290). See publication for details of 5' sequence removed.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pmIL-6 -231 was a gift from Gail Bishop (Addgene plasmid # 61287 ; http://n2t.net/addgene:61287 ; RRID:Addgene_61287)
For your References section:CD40-mediated transcriptional regulation of the IL-6 gene in B lymphocytes: involvement of NF-kappa B, AP-1, and C/EBP. Baccam M, Woo SY, Vinson C, Bishop GA. J Immunol. 2003 Mar 15;170(6):3099-108. PubMed 12626566