This website uses cookies to ensure you get the best experience. By continuing the use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
(Plasmid #61441)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61441 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRL
  • Backbone size w/o insert (bp) 12000
  • Total vector size (bp) 13237
  • Modifications to backbone
    Untranslated mRNA sequence for LacZ inserted after the MyoD stop codon to serve as an orthogonal tag for the use of single molecule mRNA in situ hybridization.
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MyoD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    959
  • GenBank ID
    NM_010866.2
  • Entrez Gene
    Myod1 (a.k.a. AI503393, MYF3, MyoD, Myod-1, bHLHc1)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro was a gift from Charles Gersbach (Addgene plasmid # 61441)
  • For your References section:

    Single-molecule analysis of myocyte differentiation reveals bimodal lineage commitment. Gibson TM, Gersbach CA. Integr Biol (Camb). 2015 Jun 8;7(6):663-71. doi: 10.1039/c5ib00057b. Epub 2015 May 8. 10.1039/c5ib00057b PubMed 25953198