LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
(Plasmid #61441)

• Purpose: Dox-inducible MyoD with mRNA tag for smFISH
• Depositing Lab: Charles Gersbach
• Publication: Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-71. doi: 10.1039/c5ib00057b. Epub 2015 May 8.

Backbone

• Vector backbone: pRRL
• Backbone size w/o insert (bp): 12000
• Total vector size (bp): 13237
• Modifications to backbone: Untranslated mRNA sequence for LacZ inserted after the MyoD stop codon to serve as an orthogonal tag for the use of single molecule mRNA in situ hybridization.
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MyoD
• Species: M. musculus (mouse)
• Insert Size (bp): 959
• GenBank ID: NM_010866.2
• Entrez Gene: Myod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)

Resource Information

• Supplemental Documents:
  • T-MyoD-ZPriP

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry