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pEX-PK-hLC3
(Plasmid #61458)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61458 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCXN2/ pCAGGS
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 9000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PK-hLC3
  • Alt name
    pHluorin-mKate2-hLC3
  • Species
    H. sapiens (human), S. pombe (fission yeast)
  • Entrez Gene
    MAP1LC3B (a.k.a. ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a)
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer GGCTTCTGGCGTGTGACC
  • 3′ sequencing primer AGCCACCACCTTCTGATAG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

We thank Dr. James Edward Rothman for providing respective pHluorin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEX-PK-hLC3 was a gift from Isei Tanida (Addgene plasmid # 61458)
  • For your References section:

    A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy. Tanida I, Ueno T, Uchiyama Y. PLoS One. 2014 Oct 23;9(10):e110600. doi: 10.1371/journal.pone.0110600. eCollection 2014. PONE-D-14-24620 [pii] PubMed 25340751