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ER-mRFP
(Plasmid #62236)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 62236 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    clontech C1
  • Backbone manufacturer
    clontech
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 4800
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ER-mRFP
  • Species
    Synthetic
  • Insert Size (bp)
    801
  • Promoter cmv
  • Tag / Fusion Protein
    • mRFP1 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer tggtttagtgaaccgt
  • 3′ sequencing primer GTTCAGGGGGAGGTGTG
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Red localization marker for mammalian endoplasmic reticulum.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ER-mRFP was a gift from Erik Snapp (Addgene plasmid # 62236 ; http://n2t.net/addgene:62236 ; RRID:Addgene_62236)
  • For your References section:

    Monitoring chaperone engagement of substrates in the endoplasmic reticulum of live cells. Snapp EL, Sharma A, Lippincott-Schwartz J, Hegde RS. Proc Natl Acad Sci U S A. 2006 Apr 25;103(17):6536-41. Epub 2006 Apr 14. 10.1073/pnas.0510657103 PubMed 16617114